( D) UMAP plots, as in ( C), with examples of read counts (in blue) for individual transcripts that define distinct iris cell types. CB, ciliary body CBE, ciliary body epithelium ECs, endothelial cells. ( C) Uniform Manifold Approximation and Projection (UMAP) representation of snRNAseq from untreated, constricted, and dilated mouse irises, with major cell types indicated. ( B) Schematic cross section of the mouse iris and ciliary body showing the locations of the major structures. ( A) Schematic cross section of a mouse eye. This work should be useful as a point of reference for investigations of iris development, disease, and pharmacology, for the isolation and propagation of defined iris cell types, and for iris cell engineering and transplantation. By immunostaining for specific iris cell types, we have observed and quantified distortions in nuclear morphology associated with iris dilation and clarified the neural crest contribution to the iris by showing that Wnt1-Cre-expressing progenitors contribute to nearly all iris cell types, whereas Sox10-Cre-expressing progenitors contribute only to stromal cells. dilated states, and (4) identified and validated antibody and in situ hybridization probes that can be used to visualize the major iris cell types. More specifically, this work has (1) defined all of the major cell types in the mouse iris and ciliary body, (2) led to the discovery of two types of iris stromal cells and two types of iris sphincter cells, (3) revealed the differences in cell type-specific transcriptomes in the resting vs. The present study provides foundational data on the mouse iris based on single nucleus RNA sequencing. It is a site of diverse ophthalmologic diseases and it is a potential source of cells for ocular auto-transplantation. The iris controls the level of retinal illumination by controlling pupil diameter.
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